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|
Gene
|
Primer
|
Sequence (5’→3’)
|
Amplicon (bp)
|
Reference
|
|---|
|
Tick rrs
|
16SF
|
F: GTATTTTGACTATACAAAGGTATTG
|
300
|
[55]
|
| |
16SR
|
R: TATTACGCTGTTATCCCTAGAGTATT
| | |
|
gltA
|
Ric-CS409d
|
F: CCTATGGCTATTATGCTTGC
|
720
|
[57]
|
| |
Ric-CS535d
|
F: GCAATGTCTTATAAATATTC
| | |
| |
Ric-CS1258n
|
R: ATTGCAAAAAGTACAGTGAACA
| | |
| |
Ric-CS2d
|
F: ATGACCAATGAAAATAATAAT
|
1200
|
[7]
|
| |
Ric-CSEndr
|
R: CTTATACTCTCTATGTACA
| | |
|
17-kDa
|
Rr17k.1p
|
F: TTTACAAAATTCTAAAAACCAT
|
450
|
[56]
|
| |
Rr17k.90p
|
F: GCTCTTGCAACTTCTATGTT
| | |
| |
Rr17k.539n
|
R: TCAATTCACAACTTGCCATT
| | |
|
Rickettsial rrs
|
Ric-16SF
|
F: GAACGAACGCTATCGGTATGC
|
1300
|
This study
|
| |
Ric-16SR1
|
R: AATTTTACCGTGGTTGGCTGC
| | |
| |
Ric-16SR2
|
R: TGCCTCTTGCGTTAGCTCAC
| | |
|
groEL
|
Ric-ESL-F1
|
F: GGTAAATGGGCAGGYACCGAA
|
1500
|
This study
|
| |
Ric-ESL-R1
|
R: GAAGCAACRGAAGCAGCATCTTG
| | |
| |
Ric-ESL-F2
|
F: ATCGTTATGAAAGAAAGCGAYG
|
1500
|
This study
|
| |
Ric-ESL-R2
|
R: AGWGCAGTACGCACTACTTTAGC
| | |
|
ompA
|
Rr190k.71p
|
F: TGGCGAATATTTCTCCAAAA
|
530
|
[56]
|
| |
Rr190k.602n
|
R: AGTGCAGCATTCGCTCCCCCT
| | |
| |
Rr190k.720n
|
R: TGCATTTGTATTACCTATTGT
| | |
- F Forward primer. R Reverse primer. Y = C or T. W = A or T
