Fig. 1

Digestion pattern of NAGT amplicon using the Acc1 enzyme in the Leishmania isolates. Lane 1: molecular weight marker (100 bp), Lane 2: undigested NAGT amplicon, Lane 3–5: positive controls (L. infantum, L. major, and L. tropica, respectively), Lane 6–8: L. infantum isolates, Lane 9: none template control