Fig. 6

Immunocytochemistry on chamber slides treated with Doxorubicin. Untreated (a, c, e, g, i) and DOX-treated (b, d, f, h, j) CIPp and CIPm ICC with graphs representing the % differences (Δ) in positivity between the controls and the DOX-treated cells for each antibody. Cell percentages were averaged over triplicate samples, and the data are expressed as the mean ± SD, asterisk represent a significant difference (p < 0.05) between controls and treated cells by two-tail Fisher exact test. ICC at 24 h treatment for P-gp in untreated (a) and DOX-treated (b) CIPp cell line. ICC for BCRP in untreated (c) and DOX-treated (d) CIPp cell line. ICC for Ki67 in untreated (e) and DOX-treated (f) CIPm cell line. ICC for TERT in untreated (g) and DOX-treated (h) CIPp cell line. ICC for p53 in untreated (i) and DOX-treated (j) CIPp cell line; multinucleated cells, indicated by the black arrow, are shown in (d) and (j) and cells with micronuclei, indicated by black arrowhead, are shown in (j). Figures at 200x magnification